• Ovulation Stimulation
  • Intra Uterine Insemination(IUI)
  • Intracytoplasmic Sperm Injection (ICSI)
  • Intracytoplasmic Morphologically Selected Sperms Injection (IMSI) and PICS
  • Blastocyst Transfer
  • Assisted Hatching
  • Vitrification or Freezing
  • Frozen Embryo Transfer (FET)
  • Donor Programme
  • Preimplantation Genetic Screening and Diagnosis Programme (PGS and PGD)
  • The Endometrial receptivity genome analysis (ERA)

Ovulation Stimulation

The medication used to stimulate the ovaries can be in the form of tablets or injections like Follicle Stimulating Hormones, this promotes the growth of a follicle within the ovary and is given daily as a subcutaneous or intramuscular injection into either the thigh or the abdomen. These commences on day 2 or 3 of your menstrual cycle or following baseline blood tests. There are many types of protocols used to have expected no. of follicles, recently individualized stimulation protocol has proven to give a better pregnancy rate.

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Intra Uterine Insemination (IUI)

IUI means placing your husband’s prepared semen sample into your uterine cavity.
The semen sample preparation is commonly known as “sperm washing”. This involves the semen sample in a test tube and then a centrifuge. This results in the collection of sperm in a “pellet” at the bottom of the test tube. The seminal fluid is removed and fluid (media) is placed above the pellet. The most active sperm will then swim up through the media. The final sample consists of the most active sperms in a small volume of media.
Depending upon the source of semen there are two types of IUI:
  • Insemination with Husband’s sperms (IUI-H)
  • Insemination with Donor’s sperms (IUI-D)
Donor sperms may be recommended when the male partner has problems with sperm quality or production, including when the sperm is of very poor quality or absence of sperms at all and as well if there is a high risk of passing on a genetic disease from the father.
Who should undergo IUI ?
Practically all infertility patients unsuccessful after trying for more than one year.It is recommended as first line of treatment to women of less than 35yrs of age

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Intracytoplasmic Sperm Injection (ICSI)

Certain couples with severe male factor Infertility cannot be helped with conventional IVF. In order to tackle this problem procedures like Micromanipulation of Oocytes & Spermatozoa have been established. ICSI is one of the most technically advanced procedure where a single spermatozoon is directly injected into the ooplasm (cytoplasm) of the oocyte. This procedure is done using an inverted microscope equipped with micromanipulators & microinjectors where a prepared sperm is injected using a microinjecting pipette (which is 60 times thinner than human hair) into the ooplasm which is held by microholding pipette (which is 40 times thinner than human hair)
The Microinjecting egg is then returned to the incubator for further culture & is checked for fertilization about 16-18 hrs after injection .The embryos thus obtained from ICSI are taken for ET following a similar manner like conventional IVF.
ICSI is indicated for the following
  • Extremely low count i.e. Oligospermia.
  • Abnormal or poor sperm morphology i.e.
  • Teratozoospermia and Globozoospermia.
  • Women with advanced age.
  • Infertility due to immunologic cause.
  • Endometriosis grade III & IV.
  • Male with ejaculatory disorders.
  • Unexplained infertility.

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Intracytoplasmic Morphologically Selected Sperms Injection (IMSI) and PICSI

Helps in magnification of the image of the sperm 7,200 times. The high magnification achieved with the IMSI objective allows us in selecting the best possible sperm for ICSI in cases of severe sperm morphology problems.
IMSI / PICSI helps improve the success rate among men with the worst prognosis and is said to be more beneficial than ICSI in patients with previous two IVF or ICSI failures. It is also useful in couples with unexplained infertility. It has been shown that IMSI resulted in better egg fertilization rates, better quality embryos and better rate of blastocyst formation.

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Blastocyst Transfer

The use of sequential stage specific media has enabled culture of Day 3 embryos to Day 5 blastocyst formation in vitro. The blastocyst is the embryonic stage at which human implantation occurs. This process of extended culture allows nature to select those embryos with the highest capacity to produce pregnancy resulting in live birth eventually. Blastocyst is an embryo which has developed for at least 5 days after fertilization & has divided into 2 different cell and central fluid filled cavity, outer cell layer is Trophoectoderm, which becomes the placental tissue in future & the inner cell mass which becomes the foetus.
Blastocyst ET gives an additional 5-10% increase in pregnancy rates than day 3 ET. It reduces the possibility of multiple pregnancy. It is also recommended to patients who have repeated day 3 ET failures, elderly women & unexplained infertility.

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Assisted Hatching

Assisted Hatching is done either by chemical method or by LASER.
How is assisted hatching performed using acid Tyrode's solution?
  1. The embryo is held with a specialized holding pipette
  2. A very delicate, hollow needle is used to expel the acidic solution against the outer "shell" (zona pellucida) of the embryo.
  3. A small hole is made in the shell by digesting it with the acidic solution.
  4. The embryo is then washed and put back in culture in the incubator.
  5. The embryo transfer procedure is done shortly after the hatching procedure.
  6. It can be done with precise laser application.
Indications For Assisted Hatching
  • Women of 35 yrs or more than 35 yrs of age.
  • Egg quality
  • Poor quality embryos (excessive fragmentation or slow rates of cell division)
  • Zona factor - embryos having a thick outer shell (zona pellucida)
  • Previous IVF failure - cases that have had one or more previous failed IVF cycles
  • Pregnancy rates for in vitro fertilization procedures with assisted hatching have been shown in some published studies to be higher than for IVF without hatching.

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Vitrification or Freezing

Vitrification is a new method of preserving oocytes (eggs) and embryos, it is an advanced rapid cell-freezing technique, allows a higher survival rate after thawing due to the fact that high concentrations of cryoprotectors are used along with reduced volumes and timings. Thus, the formation of intracellular ice crystals is prevented, which are responsible for provoking irreparable cell damage. Vitrification guarantees a survival rate of more than 80%. This technique enables the vitrified oocytes which have survived the thawing process to have similar attributes to fresh ones. They are then able to be fertilized by the spermatozoids. The generated embryos can then be implanted and develop into healthy children.
Our Center provides this cutting edge technology to our patient which allows us to freeze their remaining embryos for the further use without fear of loosing them as well we can divide their IVF cycle in to two stages to enhance further pregnancy rate and greatly reduces the stress of infertility treatment and the many personal decisions that follow. Once vitrified, oocytes and embryos may be preserved in a suspended state for years together.

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Frozen Embryo Transfer (FET)

A FET is often a good choice over a fresh stimulated cycle when a patient has frozen embryos to use. In that case, benefits include lower cost, less complex treatment (e.g. no surgical retrieval of eggs), less medication and generally similar success rates.
You may do a frozen embryo transfer (FET) cycle, whenever you have frozen embryos and wish to use them. This may be after a delivery of a child and you wish to come back and use your frozen embryos. It could also be after an unsuccessful stimulated IVF cycle, in which you have obtained frozen embryos.

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Donor Programme

Sometimes, the couple may have to resort to the donor program (either the eggs, sperms or the embryos) as the best possible option for conception.
  • Donor egg IVF successfully treats women who are
  • Carriers of genetic diseases,
  • Women who have had multiple failed cycles of IVF,
  • women with impaired ovarian function,
  • Older women
  • A poor response to fertility medications
  • Failed fertilization or form viable embryos after IVF.
As part of the IVF egg donation program protocol, an egg donor injects fertility medication to stimulate her ovaries to produce multiple eggs. Hormone replacement is used to synchronize the recipient to the egg donor cycle. Just prior to ovulation, using standard IVF techniques, the eggs are retrieved from the egg donor's ovaries and fertilized with sperm from the recipient couple and resulting embryo is transferred in the uterus.
Our donor egg IVF program has consistently had won the top pregnancy success rates for IVF egg donation (>65%).
Sperms Donar Cycle
The couple may opt for the donor sperm when the male partner is sterile (testicular failure) and therefore unable to provide a sperm sample for IVF or ICSI.In other instances, the couple may look to use a sperm donor in order to avoid transmitting a genetic disease or disorder that is carried by the male sperm.
Embryo Donar Cycle
When both partners faces infertility problems, embryo donation can be an excellent choice.
Indications for embryo donation :
  • Poor sperm production and count.
  • Problems in egg development and ovulation.
  • Attempts at other types of ART have been unsuccessful.
  • High risk of passing on genetic disorders but are unable to pursue PGD
  • Other fertility treatments are not financially feasible

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Preimplantation Genetic Screening and Diagnosis Programme (PGS and PGD)

PGS is a state-of-the-art procedure used in conjunction with in vitro fertilization (IVF) to select embryos free of chromosomal abnormalities and specific genetic disorders for transfer in to the uterus. These genetic conditions can interfere with embryo implantation, results in pregnancy loss or in the birth of a child with physical problems, developmental delay or mental retardation. PGS improves the likelihood of a successful pregnancy and birth for two distinctly different groups of patients; couples with infertility related to recurrent miscarriage or unsuccessful IVF cycles and couples who are at risk of passing on an inherited genetic disease to their offspring.
Preimplantation genetic testing may be recommended for advanced maternal age or repeated implantation failure inspite of good looking embryos.

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The Endometrial receptivity genome analysis (ERA)

ERA involves assessing a woman’s endometrial receptivity status from a molecular view point. The molecular tool allows us to diagnose whether the endometrium is receptive or not, by analysing the expression of a group of genes responsible for this function. This diagnostic method patented by iGenomix focuses on women who visit the clinic for their fertility problems. With the endometrial gene expression diagnosis, problems that may affect the embryo implantation before starting the fertility treatment can be detected. As a result, corresponding measures can be taken to carry out the fertility treatment successfully. It also enables an early diagnosis of diseases originating in the endometrium.

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Special Features of Ssmile IVF

Air Handling Unit (AHU)

AHU is a device used to condition and circulate air as part of a heating, ventilating and air-conditioning (HVAC) system. Certificate Courses in basic Infertility,USG,Embryology.

We have certified Air Handling Unit (AHU) with ISO 7 /class 10,000 (at rest) and design filtration 10+3+0.3 micron (HEPA) to have an efficiency of not less than 99.97% for 0.3µm particles, which removes particles – pollen, mould, fungal spores, dust mites, viruses, VOCs and bacteria – down to 0.3µm in size.Room pressure gradient to keep outside non sterile air away from Lab environment. The removal of these pollutants are more critical in IVF facilities, where the quality of air in laboratories and clinical procedure rooms can have enormous effects on embryo quality, embryo survival, and hence clinical outcomes of IVF treatment.Adding this facility in the LAB surely improves IVF Lab air quality and hence Pregnancy & Implantation rate consistently high.Modular IVF structure.

Technical Aspect of a Modular Lab & OT

  • All walls with ceiling are of antibacterial colors , smooth matt finish, without crevices with life of not less than ten years
  • The walls are constructed using special composite wallboard panels.
  • The juncture of wall and ceiling and any screw heads are filled with special filler and a radius is provided to all the corners to ensure easy to clean and keep hygienic.
  • The finishing of the floor of Operation Theatre is done with Static Conductive PVC.
  • The Doors are Semi Hermetic Sealing Sliding Door.
  • Air flow inside the Lab and OT is provided by a Downward Laminar Flow System.
  • A Pass Box for quick transfer of Oocytes in the lab IVF Lab
  • The ability to withstand damage by mobile equipment
  • It is impervious to moisture and unaffected by heat and steam
  • It is totally unaffected by color change,staining or mildew
  • It is capable of modification for minor alterations. Does not cause the build-up of a static electrical charges and is jointiess.
  • The finish interior looks, aesthetically pleasing and does not darken with age and cleaning.